Monasterium Laboratory obtains skin from patients undergoing routine face-lift surgery which is suitable for regeneration study in a wound healing assay using the "punch-in-a-punch" method. Skin samples are cultured ex vivo in serum-free medium for up to 7 days and test agents added to the medium (mimicking systemic delivery) or directly applied onto the epidermis at the air-liquid interphase (testing topical agent application).
During and/or after organ culture, the medium is processed for further in vitro analyses (e.g. cytotoxicity and cytokine secretion assays) and skin samples processed for in situ analyses (e.g. (immuno-) histomorphometry and in situ hybridization) or molecular analyses (e.g. qPCR, microarray, phospho-kinase assay) to investigate standard wound healing parameters, including re-epithelization and inflammation.