Monasterium Laboratory uses different microdissection techniques on hair follicles isolated from human scalp skin or follicular units to allow the routine culture of:
Hair follicles are cultured ex vivo in a serum-free defined medium to allow a large array of hair, pigmentation, cell cycle and stem cell parameters to be assessed. Besides being able to explore mechanisms of action of the test agent in question (by administering pharmacological antagonists or neutralizing antibodies), we are also experienced in gene silencing by siRNA in human skin organ culture.
Hair follicle organ culture (shown above). Upon arrival, the skin is gently shaved and placed in fresh Williams’ E medium/PBS. The skin is then cut into smaller pieces, prior to an incision at the dermal–subcutaneous fat junction. If the skin has been cut in the correct plane, a ‘lattice structure’ should be visible. HFs are then isolated with watchmakers’ forceps and placed singly or in groups into supplemented Williams’ E culture medium. Image taken from Langan et al. 2015, Exp Dematol 24:903-11.
Philpott et al. 1990; Langan et al. 2015; Olah et al. 2016; Bertolini et al. 2016; Kloepper et al. 2010; Oh et al. 2016; Purba et al. 2016; Hawkshaw et al. 2015; Hardmann et al. 2015; Ernst et al. 2013; Poblet et al. 2016; Ramot et al. 2015; Kloepper et al. 2014; Vidali et al. 2014; Samuelov et al. 2012; Kinori et al. 2012; Samuelov et al. 2013; Samuelov et al. 2012.