Human Scalp Hair Follicle Organ Culture


Monasterium Laboratory uses different microdissection techniques on hair follicles isolated from human scalp skin or follicular units to allow the routine culture of:

  • Microdissected human hair follicle
  • Full-length hair follicle
  • Entire follicular units.

Hair follicles are cultured ex vivo in a serum-free defined medium to allow a large array of hair, pigmentation, cell cycle and stem cell parameters to be assessed. Besides being able to explore mechanisms of action of the test agent in question (by administering pharmacological antagonists or neutralizing antibodies), we are also experienced in gene silencing by siRNA in human skin organ culture.

Investigating the effect of a test compound on hair
follicle growth and anagen-catagen conversion

Case study: Cyclosporin maintains anagen ex vivo

Investigating the effect of a test compound on
gray/white hair follicle pigmentation

Study example: Compound X stimulates pigmentation of white hair follicles ex vivo

Investigating the effect of a test compound on experimentally induced epithelial-mesenchymal-transition (EMT) in human hair follicles

Case study: N-acetyl-GED-0507-34-Levo (NAGED) protects and partially rescues from EMT

Hair follicle organ culture (shown above). Upon arrival, the skin is gently shaved and placed in fresh Williams’ E medium/PBS. The skin is then cut into smaller pieces, prior to an incision at the dermal–subcutaneous fat junction. If the skin has been cut in the correct plane, a ‘lattice structure’ should be visible. HFs are then isolated with watchmakers’ forceps and placed singly or in groups into supplemented Williams’ E culture medium. Image taken from Langan et al. 2015, Exp Dematol 24:903-11.

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