Human skin obtained from patients undergoing routine face-lift surgery is also suitable for regeneration study in a wound healing assay using the "punch-in-a-punch" method.
Skin samples are cultured ex vivo in serum free-medium up to 7 days and test agents are added to the medium (mimicking systemic delivery) or directly onto the epidermis at the air-liquid interphase (testing topical agent application).
During and/or after organ culture, the medium is processed for further in vitro analysis (e.g., cytotoxicity assay, cytokine secretion, ELISA), and the skin samples are processed for in situ analyses (e.g., [immuno-]histomorphometry, in situ hybridization), or molecular analyses (e.g., qPCR, microarray, phospho-kinase assay) to investigate standard wound healing parameters (incl. re-epithelization, inflammation).
As references see: