Long-Term Full-Thickness Skin Organ Culture (Skin Re-innervation)

PGP9.5/c-Kit

Until recently, full-thickness human skin organ culture faced the limitation that nerve fibers are severed and rapidly degenerate during the culture.

 

However, ML has access to the human skin re-innervation model developed by the Misery Lab, University of Brest, where full-thickness skin is co-cultured with and re-innervatived by primary rat dorsal root ganglion neurons. Moreover, ML has access to a fully humanized skin re-innervation assay that is currently under development in the Department of Dermatology, University of Münster.

 

With this model, the vitality of skin samples cultured ex vivo in serum free-medium can be substantially prolonged and test agents are added to the medium (mimicking systemic delivery) or directly onto the epidermis at the air-liquid interphase (testing topical agent application).

 

During and/or after organ culture, the medium is processed for further in vitro analysis (e.g., cytotoxicity assay, cytokine secretion, ELISA), and the skin samples are processed for in situ analyses (e.g., [immuno-]histomorphometry, in situ hybridization), or molecular analyses (e.g., qPCR, microarray, phospho-kinase assay) to investigate standard skin or hair biology parameters, including hair follicle cycle, pigmentation, hair follicle and skin regeneration (stem cell biology), immunology (incl. immune privilege), epithelial-mesenchymal transition in the stem cell compartment, neuroendocrinology and skin aging.

 

As references see:

Lebonvalet et al. 2014; Chéret et al, 2014. 

Druckversion Druckversion | Sitemap
© 2016 Monasterium Laboratory | Skin & Hair Research Solutions GmbH Center for Nanotechnology (CeNTech) | Heisenbergstraße 11 | D - 48149 Münster phone +49 (0)251 53406-460 a.borrosch@monasteriumlab.com