Until recently, full-thickness human skin organ culture faced the limitation that nerve fibers are severed and rapidly degenerate during the culture.
However, ML has access to the human skin re-innervation model developed by the Misery Lab, University of Brest, where full-thickness skin is co-cultured with and re-innervatived by primary rat dorsal root ganglion neurons. Moreover, ML has access to a fully humanized skin re-innervation assay that is currently under development in the Department of Dermatology, University of Münster.
With this model, the vitality of skin samples cultured ex vivo in serum free-medium can be substantially prolonged and test agents are added to the medium (mimicking systemic delivery) or directly onto the epidermis at the air-liquid interphase (testing topical agent application).
During and/or after organ culture, the medium is processed for further in vitro analysis (e.g., cytotoxicity assay, cytokine secretion, ELISA), and the skin samples are processed for in situ analyses (e.g., [immuno-]histomorphometry, in situ hybridization), or molecular analyses (e.g., qPCR, microarray, phospho-kinase assay) to investigate standard skin or hair biology parameters, including hair follicle cycle, pigmentation, hair follicle and skin regeneration (stem cell biology), immunology (incl. immune privilege), epithelial-mesenchymal transition in the stem cell compartment, neuroendocrinology and skin aging.
As references see: