Using different microdissection techniques, we routinely culture:
- microdissected human hair follicles
- full-length hair follicles
- entire follicular units.
Hair follicles are cultured ex vivo in a supplemented, perfectly defined, serum-free medium, and a large array of hair, pigmentation, cell cycle, and stem cell research parameters can be assessed.
After the culture, the medium is processed for further in vitro analysis (e.g., cytotoxicity assay, cytokine secretion, ELISA), and the skin samples are processed for in situ analyses (e.g., [immuno-]histomorphometry, in situ hybridization), or molecular analyses (e.g., qPCR, microarray, phospho-kinase assay) to investigate whatever hair biology parameter is relevant to the question(s) asked.
Besides being able to explore mechanisms of action of the test agent in question by administering pharmacological antagonists or neutralizing antibodies, ML is also experienced in gene silencing by siRNA in human skin organ culture.
Hair follicle organ culture. Upon arrival, the skin is gently shaved and placed in fresh Williams’ E medium/PBS. The skin is then cut into smaller pieces, prior to an incision at the dermal–subcutaneous fat junction. If the skin has been cut in the correct plane, a ‘lattice structure’ should be visible. HFs are then isolated with watchmakers’ forceps and placed singly or in groups of 3 into supplemented Williams’ E culture medium. Image taken from Langan et al. 2015, Exp Dematol 24:903-11.
As references see:
Philpott et al. 1990; Langan et al. 2015; Olah et al. 2016; Bertolini et al. 2016; Kloepper et al. 2010; Oh et al. 2016; Purba et al. 2016; Hawkshaw et al. 2015; Hardmann et al. 2015; Ernst et al. 2013; Poblet et al. 2016; Ramot et al. 2015; Kloepper et al. 2014; Vidali et al. 2014; Samuelov et al. 2012; Kinori et al. 2012; Samuelov et al. 2013; Samuelov et al. 2012.