Skin samples are cultured ex vivo in serum free-medium up to 7 days and test agents are added to the medium (mimicking systemic delivery) or directly onto the epidermis at the air-liquid interphase (testing topical agent application).
During and/or after organ culture, the medium is processed for further in vitro analysis (e.g., cytotoxicity assay, cytokine secretion, ELISA), and the skin samples are processed for in situ analyses (e.g., [immuno-]histomorphometry, in situ hybridization), or molecular analyses (e.g., qPCR, microarray, phospho-kinase assay) to investigate standard skin or hair biology parameters, including hair follicle cycle, pigmentation, hair follicle and skin regeneration (stem cell biology), immunology (incl. immune privilege), epithelial-mesenchymal transition in the stem cell compartment, neuroendocrinology and skin aging.
Besides being able to explore mechanisms of action of the test agent in question by administering pharmacological antagonists or neutralizing antibodies, ML is also experienced in gene silencing by siRNA in human skin organ culture.
As references see: