Skin barrier

We use primary epidermal keratinocytes, 2D/3D cell models, ex vivo human full thickness skin and humanized mouse in vivo models to investigate hydration and skin barrier defects and whether compounds/formulations (also via topical application) can reinforce the skin barrier. Specifically, as standardized readout parameters, we evaluate the following by immunohistology/quantitative (immuno-) histomorphometry (for our techniques, please click here).

In addition, using qRT-PCR and/or in situ hybridization, we can analyse the expression of genes involved in skin barrier integrity, and assess these within specific compartments from skin tissue sections following laser capture microdissection.

Keren et al., 2023, Riethmüller et al., 2018, Hulshof et al., 2018, Soltanipoor et al., 2018, Engebretsen et al., 2018, Engebretsen et al., 2018